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Reconstitution Guide

How to prepare lyophilized peptides with bacteriostatic water

01 What is bacteriostatic water?

Bacteriostatic water (BW) is sterile water for injection that contains 0.9% benzyl alcohol as a preservative. This compound inhibits bacterial growth, allowing the vial to be used multiple times (multi-dose) without contamination.

  • Not the same as water for injection (which is single-dose and has no preservative)
  • Not physiological saline (does not contain NaCl)
  • Not distilled water (not sterile, not suitable for injection)
Only use water that is specifically labeled for injection. Never use drinking or distilled water.

02 Materials needed

Bacteriostatic water (BW) for injection
Lyophilized peptide vial
1 mL or 0.5 mL insulin syringe (U-100)
Sterile needle 25–27G (for reconstitution)
Alcohol swabs (70% isopropyl alcohol)
Clean, well-lit surface
Dosage calculation (pre-written)

03 Reconstitution step by step

1
Prepare the environment

Wash your hands thoroughly with soap and water for at least 20 seconds. Use a clean, disinfected surface. Gather all materials before starting.

2
Disinfect the vials

Clean the rubber septum of both vials (peptide and bacteriostatic water) with an alcohol swab. Wait 30 seconds for it to dry completely.

3
Draw the bacteriostatic water

Insert the sterile needle through the rubber septum of the bacteriostatic water vial. Invert the vial and carefully draw the calculated volume.

4
Add water to the peptide vial

Insert the needle into the peptide vial septum. Direct the water toward the glass wall (never directly onto the powder) to avoid protein denaturation. Apply slowly.

5
Dissolve gently

Do NOT shake. Swirl gently in circular motions or roll the vial between your palms until the powder is completely dissolved. The solution should be clear.

6
Inspect the solution

Check that there are no visible particles. The solution should be clear and colorless (some peptides may have a slightly yellowish tint). If you see particles, do not use.

7
Label the vial

Write the date of reconstitution and the concentration (mg/mL) on the vial with a permanent marker.

8
Store properly

Store the reconstituted vial in the refrigerator at 2–8°C, away from light. See the specific storage time for each peptide.

04 Dose calculator

Enter your vial size and desired dose. The calculator recommends the optimal volume of bacteriostatic water and generates a complete dosing table in insulin units.

Reconstitution Calculator
mcg = micrograms (µg)  |  mg = milligrams  |  1 mg = 1,000 mcg
mg
e.g. 2 mg, 5 mg, 10 mg
mcg
Concentration formula
Concentration (μg/mL) = (Peptide mg × 1000) ÷ BW mL  |  μg/IU = Concentration ÷ 100

05 Injection technique (subcutaneous)

Injection sites

Abdomen (2–5 cm from navel), outer thigh, or back of arm. Rotate sites each injection.

Technique

Pinch skin and fat with 2 fingers. Insert needle at 45–90°. Release fold, inject slowly, withdraw and press for 5–10 sec.

Temperature

Take the vial out of the fridge 15–30 min before injection. Cold solutions may be more uncomfortable.

Disposal

Dispose of needles and syringes in a sharps container. Never recap used needles.

06 Storage and shelf life

Lyophilized (unopened) −20°C, up to 2 years (protected from light)
Reconstituted (refrigerated) 2–8°C, 4–8 weeks depending on peptide
Avoid Freeze-thaw cycles, direct light, room temperature >25°C
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Important reminder
This guide is for informational purposes only. Always follow the specific instructions of each peptide. Consult a healthcare professional.
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